Determination of saturated and unsaturated oligogalacturonic acids by means of thin-layer chromatography

摘要

The separation bf saturated and unsaturated oligogalacturonic acids (oligoGalAj by HPTLC technique was improved by multiple developments with 1-propanol-water (PM) mixtures on silica gel 60 plates using automatic developing chamber (ADC). The ADC allowed a standardized course of characterization. The influence of the solvent composition, the number of developments and the solvent front on the separation of the oligomers. was investigated. Saturated oligoGalA up to the nonamer and unsaturated oligoGalA up to a degree of polymerization of 13 were separated using 5 developments with PM mixtures between 7:4.50 and 7:2.75. The meta-hydroxydiphenyl reagent is proposed for the postchromatographic derivatrzation.