DIFFERENCES IN THE GLYCOSYLATION OF RECOMBINANT AND NATIVE HUMAN MILK BILE SALT-STIMULATED LIPASE REVEALED BY PEPTIDE MAPPING

摘要

The milk of some mammals contains a bile salt-stimulated lipase (BSSL). Human milk BSSL is heavily glycosylated (30-40% carbohydrate) and present at a concentration of approximately 100-200 mg/l, thereby being one of the most abundant human whey proteins. BSSL has been shown to have an important role in the uptake of energy from human milk. The risk of HIV contamination has restricted the use of banked human milk for nutritional purposes. This has evoked an interest in the production of a recombinant form of the protein for supplementation of formula.