Rapid high-performance liquid chromatographic measurement of venlafaxine and O-desmethylvenlafaxine in human plasma Application to management of acute intoxications

摘要

Venlafaxine,a second-generation antidepressant,acts by inhibition of the reuptake of presynaptic noradrenaline and serotonin.The main metabolite,O-desmethylvenlafaxine was found biologically active.For toxicological purpose,a rapid pecific and accurate RP-HPLC assay was developed for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma.A linear response was observed over the concentration range 0.2-4 #mu#g/ml.A good accuracy (<8%) was achieved for all quality controls,with inter-day and inter-day variation coefficient less than 10%.Finally,no interference was observed with other psychotic drugs encountered in acute poisoning.This rapid method(run time<10min) was used to manage four voluntary intoxications involving venlafaxine.