Feasibility of a Self-Calibrated LC/MS/UV Method to Determine the Absolute Amount of Compounds in Their Storage and Screening Lifecycle

摘要

Screening a quality compound collection against a validated drag target is a key success factor in the lead discovery process.A compound collection,ranging from thousands to over a million individual compounds,typically contains compounds made by single-compound synthesis and those by parallel synthesis.After synthesis,purification,and the purity analysis,the quantity of compounds is gravimetrically determined before they can be registered in a collection.Compounds are usually dissolved in DMSO for high-throughput screening (HTS).