Development of Fluorophore Labeled Anti-Aflatoxin Antibodies Coated SPIONs for Detection of Aflatoxin B1

摘要

Superparamagnetic iron oxide nanoparticles (SPIONs) ranging between 20-40 nm were synthesized by co-precipitation method. Functionalized SPIONs were coated with FITC-labeled-streptavidin. Biotinylated aflatoxin B1 specific anti-aflatoxin B1 antibodies were prepared, separately. The fluorophore FITC-labeled-streptavidin-SPIONs and biotinylated anti-aflatoxin B1 antibodies were allowed to interact to obtain immuno-SPIONs bioconjugates. The extract of Aspergillus flavus and Aspergillus parasiticus infected groundnut seeds was tested to detect the aflatoxin B1 molecules (<5 ng/mL) by forming immuno-SPIONs complex. The immuno-SPIONs bioconjugates were characterized and evaluated by gel electrophoresis, Fourier transform infrared spectroscopy, Fluorescence microscopy and Transmission electron microscope using appropriate controls. The bioconjugated FITC-labeled-STV-SPION particles were found to be stable over 2 months at 4 ℃.