Reduced protein adsorption and platelet adhesion by controlled variation of oligomaltose surfactant polymer coatings

摘要

A series of oligomaltose surfactant polymers were prepared by the simultaneous coupling of hydrophilic maltonolactone (of 2 (M2), 7 (M7), or 15 (M15) glucose units) and hydrophobic N-(hexanoyloxy)succinimide (Hex) groups to the amino groups of a poly(vinyl amine) backbone. The surfactants were characterized by FTIR and ~1HNMR spectroscopies for purity and composition. Contact-angle and AFM measurements confirmed full monolayer adsorption for all surfactants on a model surface, highly oriented pyrolytic graphite, while full coverage was observed on polyethylene only for PVAm (M7:Hex) due to the optimal M7:Hex ratio and Hex chain density. On graphite, protein resistance increased with increasing coating thickness from 81.4 to 85.8 to 95.8 percent for the M2, M7, and M15 surfactants, respectively. Additionally, static platelet adhesion on all three surfactants dropped substantially to 15 percent (M2), 17 percent (M7), and 16 percent (M15) compared to glass (adhesion normalized to 100 percent) and a polyurethane (24 percent) surface. Protein- and platelet-resistant properties of the controlled oligomaltose layers are discussed by analysis of molecular modeling, oligomaltose and hexanoyl chain densities, and surfactant stability.