Enzymatic production of L-citrulline by hydrolysis of the guanidinium group of L-arginine with recombinant arginine deiminase

摘要

In this study, a simple, efficient enzymatic production process for the environmentally friendly synthesis of L-citrulline from L-arginine was developed using arginine deiminase (ADI) from Lactococcus lactis. Following overexpression of L. lactis ADI in Escherichia. coli BL21 (DE3) and experimental evolution using error-prone PCR, mutant FMME106 was obtained with a K-m for L-arginine of 3.5 mM and a specific activity of 195.7 U/mg. This mutant exhibited a maximal conversion of 92.6% and achieved a final L-citrulline concentration of 176.9 g/L under optimal conditions (190 g/L L-arginine, 15 g/L whole-cell biocatalyst treated with 2% isopropanol for 30 min, 50 degrees C, pH 7.2, 8h). The average L-citrulline synthesis rate of 22.1 g/L/h is considerably higher than that reported for other similar biocatalytic approaches, therefore the process developed in the present work has great potential for large-scale production of L-citrulline. (C) 2015 Published by Elsevier B.V.