肽酰基精氨酸脱亚氨酶-4、抗环瓜氨酸肽抗体联合检测在类风湿关节炎诊断中的应用价值

摘要

目的：：研究肽酰基精氨酸脱亚胺酶-4(PADI-4)、抗环瓜氨酸抗体(抗CCP抗体)联合检测在类风湿关节炎(RA)诊断中的价值。方法：用酶联免疫吸附试验双抗体夹心法检测58例RA患者( RA组)、40例其他风湿性疾病患者(非RA组)和30名健康志愿者(健康对照组)的血清PADI-4、抗CCP抗体、抗聚角蛋白傲丝蛋白抗体( AFA)、抗突变型瓜氨酸波形蛋白抗体(抗MCV抗体)和抗RA33抗体的浓度,并对其受试者工作特征曲线、灵敏度、特异度、相关性和诊断效能进行分析。结果：RA患者血清中PADI-4、抗CCP抗体、AFA、抗MCV抗体和抗RA33抗体的浓度均显著高于非RA组和健康对照组(P0.05)。在多种抗体联合检测中,PADI-4、抗CCP抗体均为阳性诊断RA的特异度高达100.0%,单项检测PADI-4为91.4%,抗CCP抗体为95.7%。结论：PADI-4、抗CCP抗体联合检测能提高RA的特异性和灵敏度,有助于对RA的诊断。%Objective:To investigate the value of the combined detection of peptidylarginine deaminase-4 ( PADI-4 ) and anti-cyclic citrullinated peptide(anti-CCP) antibody in the diagnosis of rheumatoid arthritis(RA). Methods:The levels of PADI-4,anti-CCP antibody,anti-MCV antibody, AFA antibody and anti-RA33 antibody in 58 patients with RA ( RA group ) , 40 patients with other rheumatoid disease ( non-RA group ) and 30 healthy people ( control group ) were detected by ELISA. The ROC curve, sensitivity, specificity,relevance and diagnostic effectiveness in all cases were analyzed. Results:The serum levels of PADI-4,anti-CCP antibody, anti-MCV antibody,AFA antibody and anti-RA33 antibody in RA patients were significantly higher than those in non-RA group and control group(P0. 05). Among the combined detection of multiple antibodies, the specificity of the PADI-4 and anti-CCP antibody in the diagnosis of RA was 100. 0%,and the positive detection of PADI-4 and anti-CCP antibody were 91. 4% and 95. 7%, respectively. Conclusions:The combined detection of PADI and anti-CCP antibody can improve the specificity and sensitivity of RA,which can help the diagnosis of RA.