Subtilisin-catalyzed peptide synthesis using carbamoylmethyl esters as acyl donors

摘要

We have recently found that the carbamoylmethyl (Cam) ester acts as an excellent acyl donor in the kinetically controlled peptide-bond formation mediated by alpha-chymotrypsin [1].Thus,major drawbacks associated with protease-catalyzed peptide synthesis-narrow substrate specificity and secondary hydrolysis of a growing petpide-can be overcome by employing this ester.