Bioelectrocatalytic sensor for triglycerides in human skin sebum based on enzymatic cascade reaction of lipase, glycerol kinase and glycerophosphate oxidase

摘要

We report the development of an electrochemical biosensor for the quantification of triglycerides inhuman skin sebum, based on a multienzyme cascade reaction. The presence of excessive triglycerides inhuman sebum is one of the leading causes of various skin ailments. However, to the best of our knowledge, no bioelectrocatalytic approach for the quantification of sebum triglycerides has been made. Inorder to develop triglyceride biosensor, we fabricated a multienzyme- associated electrode incorporatinglipase, glycerol kinase, and glycerophosphate oxidase. Enzymes were deposited by electrostatic forceand further stabilized via crosslinking between enzymes and polymer matrices. The enzyme- modifiedbiosensing electrode maintained its bioelectrocatalytic activity for five days. An additional constraint wasthe limited solubility of sebum triglycerides in aqueous electrolytes, impeding the analysis. To addressthis issue, triglyceride samples were prepared in the form of micelles, enabling efficient sample preparation for biosensor signaling. Calibration tests revealed that the designed assay had a detection rangeof 15- 200 mg/ dL of micellar triglyceride, which covered the required determination range. The developed biosensing approach was successfully used to determine triglyceride concentrations in real sebumsamples of unknown triglyceride content. (C) 2014 Elsevier B. V. All rights reserved.