Optimizing the fabrication processes for manufacturing a hybrid hierarchical polyurethane-cell/hydrogel construct

摘要

It is essential to control the overall composition and internal architecture for complex organ manufacturing. In this study, several subprocesses were optimized to produce hybrid hierarchical polyurethane-cell/hydrogel constructs with an intrinsic network of grid and branched channels using a double-nozzle low-temperature deposition rapid prototyping system. The formation quality was mainly determined by the polymer concentration and composition. However, the cell viability was mainly determined by the formation time. Cell sensitivities to the inner nozzle diameter and extrusion flux were not significantly different within the given parameter ranges. The integrity of the two material systems can be varied by the formation routes and layer thickness. Under the optimal fabrication parameters, such as formation time within 20 min and gelatin:alginate:fibrinogen ratio of 2:1:1, a high cell survival rate of 80% was attained. The design and fabrication strategies used to create such a complex heterogeneous objects directly from a computer-aided design model represent a promising route for robotic hybrid hierarchical construct implementations, which would allow easy expansion of the subprocessing capabilities and scale up manufacturing capabilities.