New Polysaccharide-based Microparticles Crosslinked with Siloxane:Interactions with Biologically Active Substances

摘要

The interaction of microparticles of carboxymethyl pullulan crosslinked with siloxane (provided by a new crosslinking agent:3-(glyeid oxypropyl) trimethoxysilane) with biologically active molecules,such as enzymes (lysozyme) and drugs (propranolol,quinidine) was studied.The anionic amphiphilic supports retained through electrostatic and/or hydrophobic forces,variable amounts of the substances as a function of their structure,such as crosslinking degree and amount of uncrosslinked alkylsilane chains.The absorption of lysozyme on the supports followed the Langmuir isotherm,which allowed the calculation of constants k_1 and k_2.Both retention and in vitro release behavior of these support potential applications in controlled drug release as well as immobilization and purification of enzymes.