Development of dipsticks for simultaneous detection of Vip3A and Cry1 Ab/Cry1 Ac transgenic proteins.

摘要

The number of GM crops being cultivated and amounts of GM produce reaching the market are increasing every yr. The transgenes (vip3A, cry1Ab and cry1Ac) from Bacillus thuringiensis are used by plant breeders for development of insect pest-resistant GM crops. It is therefore necessary to develop an easy, rapid and reliable detection assay to discriminate between GM crops and non-GM crops. Dipstick strips using colloidal gold-labeled polyclonal antibodies were developed for simultaneous detection of Vip3A and Cry1Ab/Cry1Ac proteins. The assay was essentially based on the sandwich format of immunoassay, which was completed within 10 min, and the results were evaluated visually. Detection limits were 50 ng/ml (50 ppb) for both Cry1Ac and Cry1Ab proteins, and 100 ng/ml (100 ppb) for Vip3A protein. The developed dipsticks are suitable for on-site simultaneous screening of GM crops bearing 2 proteins, which, in turn, reduce cost and time of the assay.