DIAGNOSTIC KIT BASED ON POLYMERASE CHAIN REACTION FOR DETECTION OF CRY1 AC GENE IN BT COTTON BOLLGARD-I.

摘要

The present invention comparising a process enabling detection of transgene i.e. cryAc gene responsible for production of cry1Ac protein for insert resistance in Bt cotton bollgard-1 using Polymerase Chain Reaction. the process comparises designing of the primer sequence for cry1Ac gene, Preparation of polymerase chain reation mixture, amplification of target sequence using polymerase chain reaction with three temperatures regime and conversion of the technolgy into form of a kit.