Determination of total folates in infant formula and adult nutritionals by trienzyme extraction and UPLC-MS/MS quantitation: First Action 2011.06.

摘要

The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) to determine total folates in infant formulas and adult nutritionals after trienzyme digestion. Deconjugation of the various folate polyglutamates to folate monoglutamates is achieved by using rat plasma conjugase after sample digestion with proteinase and alpha-amylase during the trienzyme digestion process. This method shows linearity of folate concentrations in the range 10-19 100 mug/100 g. Extension of the range to cover folate concentrations of 5-2 000 000 mug/100 g can be achieved with appropriate adjustment of the sample weight and SPE cleanup loading volume. The recoveries ranged from 94.10 to 101.34%.