QuickTox (TM) Kit for Quick Scan DON (Vomitoxin)

摘要

The QuickTox Kit for QuickScan DON (Vomitoxin) uses lateral flow technology and a reader-based system for the quantification of deoxynivalenol (DON, vomitoxin) residues in cereal grain commodities. The present work was performed to obtain AOAC Research Institute Performance Tested Method(SM) certification for testing wheat, maize, wheat bran, wheat flour, and barley samples with DON contamination levels as high as 5 ppm. Assay performance was examined using naturally contaminated and spiked samples in internal and independent laboratory evaluations and was compared to previously established acceptance criteria. Performance was evaluated with direct regard to linearity, matrix, selectivity, robustness, and stability. All data points in all studies were within the ranges defined in the acceptance criteria. The assay exhibited a linear dose response over the range tested, 0-5.0 ppm, with R-2 values exceeding 0.97. RSD of repeatability, or RSD values for results ranged from 3.12 to 16.01% across all tested commodities and levels. Assay results were not affected by the presence of other common mycotoxins: aflatoxin Bl, fumonisin Bl, ochratoxin A, or zearalenone. Selectivity for modified DON was examined, specifically 15-acetyl DON, 3-acetyl DON, DON-3-glucoside, and another tricothecene, nivalenol; all were detected. The assay produced acceptable results in robustness studies when assay timing, temperature, and volume were co-varied. The QuickTox Kit for QuickScan DON (Vomitoxin) assay is a convenient and reliable method for determination of DON in grain commodities.