DETERMINATION OF FORMETANATE HYDROCHLORIDE IN STRAWBERRIES

摘要

A method was established for determining formetanate hydrochloride in strawberries by reversed-phase high-performance liquid chromatography (HPLC). Formetanate hydrochloride was extracted from strawberries with acidified organic solvents (acetonitrile:HCl:ethyl acetate), and the extract was purified with a solid-phase extraction (SPE) column followed by a strong cation-exchange (SCX) cartridge, and analyzed by HPLC with UV diode array detection at lambda m of 254 nm. The mobile phase was 0.01 M, pH 8' ammonium phosphate buffer:acetonitrile (70:30). Formetanate was quantitated by the internal standard method. The percent recovery averaged 87.4 +/- 2.2 (0.167 mg kg(-1)), 89.0 +/- 2.1 (1.67 mg kg(-1)), and 91.9 +/- 3.2 (16.7 mg kg(-1)), the limit of determination (LD) was 0.018 mg kg(-1), and the limit of quantitation (LQ) was 0.18 mg kg(-1).