首页> 外文期刊>Journal of Agricultural and Food Chemistry >In Vitro Anti-inflammatory and Anti-proliferative Activity of Sulfolipids from the Red Alga Porphyridium cruentum.
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In Vitro Anti-inflammatory and Anti-proliferative Activity of Sulfolipids from the Red Alga Porphyridium cruentum.

机译:赤藻卟啉菌中硫磺脂质的体外抗炎和抗增殖活性。

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A sulfoglycolipidic fraction (SF) isolated from the red microalga Porphyridium cruentum was analyzed for fatty acid composition and assayed for ability to inhibit, in vitro, the generation of superoxide anion in primed leucocytes and the proliferation of a panel of human cancer cell-lines. Results demonstrated that SF contained large amounts of palmitic acid (26.1%), arachidonic acid (C20: 4omega-6, 36.8%), and eicopentaenoic (C20:5omega-3, 16.6%) acids, and noticeable amounts of 16:1n-9 fatty acid (10.5%). It strongly inhibited both the production of superoxide anion generated by peritoneal leukocytes primed with phorbol myristate acetate (IC(50): 29.5 &mgr;g/mL), and the growth of human colon adenocarcinoma DLD-1 and to a lesser extent of human breast adenocarcinoma MCF-7, human prostate adenocarcinoma PC-3, and human malignant melanoma M4 Beu cell-lines, and therefore might have a chemopreventive or chemotherapeutic potential, or both. It was found markedly more cytotoxic than sulfoquinovosyldiacylglycerols from plant used as a standard (STD), due to a stronger ability to inhibit DNA alpha-polymerase (IC(50): 378 &mgr;g/mL, vs 1784 &mgr;g/mL for STD). After a 48-h continuous treatment, IC(50) values for growth inhibition were in the range of 20-46 &mgr;g/mL instead of 94 to >250 &mgr;g/mL for STD, and those for inhibition of metabolic activity were in the range of 34-87 &mgr;g/mL instead of >250 &mgr;g/mL for STD. The higher anti-proliferative effect was observed on colon adenocarcinoma DLD-1 cells, and the weaker effect was observed on breast adenocarcinoma MCF-7.
机译:分析了从红色微藻红景天(Porphyridium cruentum)分离出的巯基糖脂级分(SF)的脂肪酸组成,并测定了在体外抑制引发白细胞中超氧阴离子的生成和一组人类癌细胞系增殖的能力。结果表明,SF包含大量的棕榈酸(26.1%),花生四烯酸(C20:4omega-6,36.8%)和二十碳五烯酸(C20:5omega-3,16.6%)酸,以及明显的16:1n- 9个脂肪酸(10.5%)。它强烈抑制由佛波肉豆蔻酸酯乙酸盐(IC(50):29.5μg/ mL)引发的腹膜白细胞产生的超氧阴离子的产生以及人结肠腺癌DLD-1的生长以及对人乳腺的抑制腺癌MCF-7,人前列腺腺癌PC-3和人恶性黑色素瘤M4 Beu细胞系,因此可能具有化学预防或化学治疗的潜力,或两者兼具。由于具有更强的抑制DNAα聚合酶的能力,发现它比起用作标准品(STD)的植物中的磺基喹喔基二酰基甘油具有更高的细胞毒性(IC(50):378μg/ mL,而1784μg/ mL)。 STD)。连续治疗48小时后,抑制性生长的IC(50)值在20-46μg/ mL范围内,而不是STD从94到> 250μg/ mL,抑制代谢活性的值STD的浓度范围是34-87 mg / mL,而不是250 mg / g。对结肠腺癌DLD-1细胞观察到较高的抗增殖作用,而对乳腺癌MCF-7观察到较弱的作用。

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