Determination of fragrance allergens in essential oils and evaluation of their in vitro permeation from essential oil formulations through cultured skin

摘要

We developed a high-performance liquid chromatography method using fluorometric detec- tion to simultaneously determine the allergens in fragrances, primarily structurally related allylbenzenes in essential oils. The effect of essential oil carriers-cloves, basil, and cinnamon-on the release and per- cutaneous absorption of the oils was studied in vitro using a cultured epidermal autograft membrane model.