A diagnostic method for herpes simplex keratitis by simultaneous measurement of viral DNA and virus-specific secretory IgA in tears: an evaluation

Shoji Jun; Sakimoto Tohru; Inada Noriko; Kamei Yuko; Matsubara Masao; Takamura Etsuko; Sawa Mitsuru

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A diagnostic method for herpes simplex keratitis by simultaneous measurement of viral DNA and virus-specific secretory IgA in tears: an evaluation

机译：同时测量眼泪中病毒DNA和病毒特异性分泌型IgA的单纯疱疹性角膜炎诊断方法：一项评估

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We performed simultaneous measurement of herpes simplex virus (HSV) DNA by real-time polymerase chain reaction (real-time PCR) and of HSV-specific secretory IgA antibody (HSV-sIgA) by enzyme-linked immunosorbent assay (ELISA) in tears obtained using Schirmer strips in order to investigate its diagnostic efficacy for herpes simplex keratitis (HSK).

机译：我们通过实时聚合酶链反应（real-time PCR）同时测量单纯疱疹病毒（HSV）DNA和通过酶联免疫吸附测定（ELISA）在获得的眼泪中对HSV特异性分泌型IgA抗体（HSV-sIgA）进行同时测量为了研究其对单纯疱疹性角膜炎（HSK）的诊断功效，使用了Schirmer条。

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来源
《Japanese Journal of Ophthalmology》 |2016年第4期|共8页
作者
Shoji Jun; Sakimoto Tohru; Inada Noriko; Kamei Yuko; Matsubara Masao; Takamura Etsuko; Sawa Mitsuru;
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正文语种 eng
中图分类眼科学;
关键词
Herpes simplex virus; Keratitis; Real-time polymerase chain reaction; Secretory IgA; Tear fluid;

机译：单纯疱疹病毒角膜炎实时聚合酶链反应分泌型IgA泪液;

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专利

1. A diagnostic method for herpes simplex keratitis by simultaneous measurement of viral DNA and virus-specific secretory IgA in tears: an evaluation [J] . Shoji Jun, Sakimoto Tohru, Inada Noriko, Japanese Journal of Ophthalmology . 2016,第4期

机译：同时测量眼泪中病毒DNA和病毒特异性分泌型IgA的单纯疱疹性角膜炎诊断方法：一项评估
2. The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA [J] . Qiu Jini, Huang Feifei, Wang Zhujian, Graefe's archive for clinical and experimental ophthalmology: Albrecht von Graefes Archiv fur klinische und experimentelle Opthalmologie . 2017,第7期

机译：撕裂HSV-SIGA和HSV-DNA组合对基质疱疹异位角膜炎诊断效率的评价
3. Evaluation of tear samples for Herpes Simplex Virus 1 (HSV) detection in suspected cases of viral keratitis using PCR assay and conventional laboratory diagnostic tools. [J] . Satpathy G, Mishra AK, Tandon R, British journal of ophthalmology . 2011,第3期

机译：使用PCR测定法和常规实验室诊断工具评估可疑病毒性角膜炎病例中的泪液样本，以检测单纯疱疹病毒1（HSV）。
4. Evaluation of viral load and indication of DNA CMV, EBV herpes viruses in frequently ill children [C] . Kozlova S.N., Savelyeva E.V. World Asthma COPD Forum . 2011

机译：评估病毒载荷和DNA CMV的指示，常生儿童中的EBV疱疹病毒
5. Evaluation of Two Novel Antiviral Peptides in a Murine Model of Herpes Simplex Virus Type 1 Keratitis [D] . Jose, Gilbert G. 2012

机译：在单纯疱疹病毒1型角膜炎的小鼠模型中评估两个新型抗病毒肽。
6. Secretory IgA specific for herpes simplex virus in lacrimal fluid from patients with herpes keratitis--a possible diagnostic parameter. [O] . B Pedersen, S Møller Andersen, A Klauber, 1982

机译：疱疹性角膜炎患者泪液中的单纯疱疹病毒特异性分泌型IgA-一种可能的诊断参数。
7. Secretory IgA specific for herpes simplex virus in lacrimal fluid from patients with herpes keratitis--a possible diagnostic parameter. [O] . Pedersen, B, Møller Andersen, S, Klauber, A, 1982

机译：疱疹性角膜炎患者泪液中的单纯疱疹病毒特异性分泌型IgA-一种可能的诊断参数。

A diagnostic method for herpes simplex keratitis by simultaneous measurement of viral DNA and virus-specific secretory IgA in tears: an evaluation

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