Recombinant DNA inserted with herpes simplex virus gene, mammalian cells transformed with said recombinant DNA, and method for the production of herpes simplex virus proteins

摘要

Novel recombinant DNA obtained by inserting a herpes simplex virus gene (e.g. HSVgB) into an E. coli plasmid inserted with TK gene, novel transformed mammalian cells obtained by transforming mammalian cells, particularly TK^- mammalian cells, with said recombinant DNA, and method for the production of herpes simplex virus proteins (e.g. g8) in a high yield and high purity by culturing said transformed mammalian cells. The herpes simplex virus proteins are useful for the preparation of HSV vaccine and diagnostic reagents for herpes simplex infections.