Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex intergeneric Cross

摘要

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of [C. grandis (1.) Osb. X [C. paradisi Macf. X Poncirus trifoliate (L.) Raf.]] X {[(C. paradisi Macf. x P. trifoliate (L.) Raf.) X C. reticulata Blanco] X [(C. paradisi Macf. X Poncirus trifoliate (L.) Raf.) X C. sinerisis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributedinto 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.