Changes in the Level of Serum High-Temperature Requirement A1 (HtrA1) during pregnancy and its relationship to preeclampsia

摘要

Objective. To investigate the expression and changes of high-temperature requirement A1 (HtrA1) during pregnancy and the use of this value in predicting preeclampsia. Method. Serum samples were collected from pregnant mothers at different gestational weeks, and double-antibody sandwich enzyme-linked immunosorbent assay was employed to describe the changes in HtrA1 in serum during pregnancy. Results. (i) In Xi'an area of China, the incidence of preeclampsia was 4.95, including 0.85 of early-onset type and 4.10 of late-onset type; (ii) the HtrA1 showed a lognormal distribution during pregnancy in the maternal serum, with the peak at 1720 weeks of pregnancy; (iii) the HtrA1 levels in preeclampsia mothers peaked at 1316 weeks of pregnancy, followed by acute decline until 2124 weeks, then remained stable; (iv) the HtrA1 levels in preeclampsia mothers were higher than the control group in 1316 weeks and lower than that in 2124 weeks (p < 0.05); (v) the criteria using lgHtrA1 level at 1.684 during 1316 weeks of pregnancy could predict hypertension disorders complicating pregnancy (HDCP) with sensitivity of 62.1 and specificity of 53.7. This could be improved to 85 and 83, respectively, when combined with body mass index as well as education background of the mother. Conclusion. (i) HtrA1 showed lognormal distribution during pregnancy for all populations; (ii) HtrA1 level peaked at 1720 weeks in normal group of pregnant mothers, and the increase of HtrA1 level in 1316 weeks could predict the risk of preeclampsia; (iii) the risk calculation formula for preeclampsia: p () eY/(1 eY) (Y -15.87 3.706 × lgHtrA1 0.134 × mean arterial pressure (MAP) - 1.4 × education level code); e = 2.718.