Production of tannase by Aspergillus niger HA37 growing on tannic acid and olive mill waste waters.

摘要

Production of tannase (EC 3.1.1.20) by Aspergillus niger HA37 on a synthetic culture medium containing tannic acid at different concn. or olive oil mills effluents was studied. Max. enzymic activity increased with increasing initial concn. of tannic acid (respectively, 0.6, 0.9 and 1.5 U/ml in medium containing 0.2, 0.5 and 1% tannic acid). Tannase production achieved by A. niger HA37 using 4-fold diluted olive oil mill waste water (OMWW) as substrate, was between 0.37 and 0.65 U/ml. Enzyme production on the diluted OMWW remained stable for >30 h. Growth of A. niger HA37 on OMWW correlated with about 70% degradation of phenols present in the waste. It is concluded that this strain has the capacity to degrade complex waste waters which may cause environmental damage to aquatic streams.