STRUCTURAL STUDIES OF O-SPECIFIC POLYSACCHARIDE CHAINS OF THE LIPOPOLYSACCHARIDE FROM YERSINIA ENTEROCOLITICA SEROVAR 0/10

摘要

Lipopolysaccharide (LPS) was isolated from Yersinia enterocolitica serovars O:10 and O:10 KL and the structural pattern of O-specific sugar chains elucidated. The rhamnan and L-xylulose (L-threo-pent-2-ulose) as constituents of the O-specific polysaccharide were obtained by autohydrolysis of the LPS. The rhamnan was shown to be a linear, alpha-(1 --> 3)-linked polysaccharide in the D configuration. L-Xylulose was purified using paper chromatography on a preparative scale and its structure was confirmed by C-13 NMR spectroscopy. Using sugar and methylation analysis and C-13 NMR spectroscopy of the LPS and the rhamnan, the structural features of the disaccharide repeating unit of the Y. enterocolitica O:10 O-specific polysaccharide were elucidated as: ?GRAPHICS? [References: 11]