STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L, PKKK(P) 679D - PRODUCER OF MONOCLONAL ANTIBODY WITH SPECIFICITY TO PROTEIN-POLYSACCHARIDE ANTIGEN FROM YERSINIA ENTEROCOLITICA O3 AND O9 SEROVARS

摘要

FIELD: biotechnology, hybridoma technology.;SUBSTANCE: hybridoma strain is prepared by fusion of murine plasmocytoma Sp2/0-Ag.8 and B-lymphocytes of murine spleen of the inbred strain BALB/c immunized with protein-polysaccharide complex from Y. enterocolitica. Hybridoma produces monoclonal antibodies of isotype IgG to Y. enterocolitica O3 and O9 serovars used as components of IFA-test-system for identification of indicated serovars that are isolated most often in European areas from sick humans, agricultural animals and from objects of environment. The usage of monoclonal antibodies producing by hybridoma allows carrying out the identification of Y. enterocolitica strains of indicated serovars representing the most epidemic danger among other intestine-persistent microorganisms. Invention can be used in the development of diagnostic test-systems for identification of Y. enterocolitica strains O3 and O9 serovars for aims laboratory diagnosis in the public health, veterinary science and in carrying out scientific investigations.;EFFECT: valuable properties of strain.;1 tbl, 2 ex