Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes

摘要

A novel water-soluble heteropolysaccharide FVP60-B was extracted from the fruiting bodies of Flammulina velutipes with boiling water and purified by Sephacryl S-300 and S-400, which molecular weight was estimated to be 1.3 x 10~4 Da by HPLC. It is composed of L-fucose, D-mannose, D-glucose and D-galactose in a ratio of 1.16:0.82:1.00:3.08. Sugar analysis, methylation analysis together with ~1H, ~(13)C and 2D NMRspectroscopy disclosed that FVP60-B is consisted of a α-(1 → 6)-D-galactopyranan backbone with a terminal fucosyl, terminal glucosyl and α-(1 → 6)-D-mannopyranan units on O-2 of 2,6-O-substituted-D-galactosyl units.