Effects of temperature and medium composition on pollen germination of 'Bengal' and 'Chakrapat' lychee (Litchi chinensis Sonn.) in vitro.

摘要

We determined favorable temperatures for in vitro germination of 'Bengal' and 'Chakrapat' lychee pollen, and quantified effective sucrose, boric acid, and agar concentrations for 'Chakrapat' germination medium. Pollen germination was high at 25-30 degrees C in 'Bengal' and at 25-35 degrees C in 'Chakrapat', and pollen tube elongation proceeded well at 25-30 degrees C in both cultivars. The pollen germination percentage and pollen-tube elongation in 'Chakrapat' were highest on 1% agar media containing 15% sucrose and 10 ppm boric acid when incubated at 25 degrees C. Pollen germination continued until 12 h after placement on media, and vigorous pollen-tube elongation continued until 9 h after placement.