Adherence and slime production in coagulase negative staphylococci isolated from different animal sources

摘要

The aim of this study was to determine slime production and the adherence of coagulase negative staphylococci (CNS) strains isolated from cows' milk with mastitis and chickens which have pathological disorders. For this purpose, 60 CNS strains isolated from cows' milk with mastitis and 60 from chickens suffering from skin and joint diseases were examined. In addition, 20 strains isolated from healthy cows and healthy chickens were used as control groups. Slime production was conducted by the tube method and the results were recorded as -, +, ++ and +++ after 24 h incubation according to the formation of a film layer on the tube surface (inside walls) and the thickness of the film layer. Adherence was determined by spectrophotometry. Adherence measurements were repeated three times to obtain a median OD value. According to the results, bacteria were grouped in three categories: nonadherent, weakly adherent and strongly adherent. Comparisons of our findings were performed through chi-square test. In conclusion, pathogenic CNS strains isolated from the milk of cows with mastitis and lesions of chickens were more slime productive and adherent compared to strains isolated from healthy animals. This difference between the two groups was found to be clinically significant (P < 0.001 and P < 0.05, respectively) and led us to conclude that slime productivity and adherency may be related to a disease caused by CNS strains.