赤水丹霞山土壤来源抗菌活性链霉菌的分离及放线菌素产生链霉菌Streptom ycessp.C SD X 001发酵产物分析

摘要

分离、鉴定赤水丹霞地区土壤来源的抗临床耐药菌活性的链霉菌。采集赤水丹霞山区不同海拔的土样并利用多种培养基分离、纯化菌株。热酚法提取菌株基因组DNA，PCR扩增16SrRNA基因并测序后在线比对以确定其系统分类学地位。利用多种测试菌测试抗菌活性，通过HPLC和LC/MS分析菌株发酵抽提物并与数据库比较。分离得到菌株600株，其中59株链霉菌中有41株具有1种以上的抗测试菌活性。Streptomycetessp. CSDX001具有抗耐药耐甲氧西林金黄色葡萄球菌、抗耐万古霉素金黄色葡萄球菌、抗多药耐药鲍曼不动杆菌、抗枯草芽孢杆菌、抗藤黄微球菌、抗白色念球菌的活性，具有抗多种微生物的活性和产生多种新结构次级代谢产物潜力，可能是抗微生物新天然活性产物的重要来源。%In orderto isolate and identify the Streptom ycetesspp.w ith antim icrobialactivity from C hishuiD anxia region,the soilsam plesfrom differ-entaltitudes w as collected,and then isolated and purified by a variety ofm edium .The strain gene D N A w as extracted by hotphenolm ethod,16S rR N A PC R products w as sequenced and blasted online for determ ine the phylogeny ofisolated strains.A ntim icrobialactivities w ere detected w ith m ultiple test bacteria,and the ferm entation extracts w ere analyzed by H PLC and LC /M S,and the results w ere com pared w ith database.R esults show ed that600 strains w ere isolated and 41 outof59 Streptom ycetes strains had m ore than one antim icrobialactivity.Streptom yces sp.C SD X 001 show ed resistance activity to m ethicillin-resistantStaphylococcus aureus,vancom ycin-resistantStaphylococcus aureus,m ultidrug resistantA cineto-bacter baum annii,B acillus subtilis,M icrococcus luteus,C andida albicans;itshow ed antim icrobialactivities and secondary m etabolites-producing potential,w hich m ightbe a potentialbacterialresource foractive naturalproductsresearch and developm ent.