Oxidative stress following extreme physical stress. Part 1: Genesis, clinical relevance and studies on vitamin E-supplemented sled dogs

摘要

An experimental study was performed in order to investigate oxidative stress in sled dogs after short races, which can be ameliorated by supplementation of the antioxidant vitamin E. Potentially occurring organ damages should be recorded in order toassess the clinical relevance. Therefore in blood samples, drawn before and after the race, the antioxidative status (TEAC, vitamin C, vitamin E), a radical marker (HNE-modified proteins) and resulting organ damages (organspecific enzymes) were measured. The consumption of antioxidants (decrease of TEAC, vitamin C and E) indicates a significant oxygen radical production of the dogs during the race, confirmed by an increase of the radical marker HNE-mod. proteins. The rise of CK- and AST activity provesthe damage of skeletal muscles. The less pronounced rise of CK- and AST activity in the vitamin E-supplemented dogs supports the hypothesis, that during extreme physical stress a significant amount of oxygen radicals is liberated and especially damagesin skeletal muscles can be ameliorated by the antioxidant vitamin E.