A gene-specific primer extension and liquid bead array system for killer cell immunoglobulin-like receptor genotyping.

摘要

We present a simple and accurate method for killer cell immunoglobulin-like receptor (KIR) genotyping using KIR genes-specific primer extension (GSPE) followed by bead array hybridization (GSPE method). The results were 100% concordant with the International Histocompatibility Working Group (IHWG) reference typing information. In addition, GSPE method can obtain results in 8 h from DNA with 10 ng genomic DNA in a 96 well-based assay format. Human natural killer (NK) cells are bone-marrow-derived lymphocytes that are crucial components of the innate immune system. NK cell functions are controlled by diverse families of antigen receptors and host cells expressing major histocompatibility complex (MHC) class I antigen. The genes encoding KIRs, a family of genes clustered in 19q3.4, are prominent among NK cell receptors (1).