KIR (KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR) TYPING METHOD USING GSPE(GENE SPECIFIC PRIMER EXTENSION)

摘要

PURPOSE: a kind of isolation Natural Killer Cell Receptors genotype is provided using gene specific amplification technique makes quality analysis. ;CONSTITUTION: a kind of isolation Natural Killer Cell Receptors genotype includes: that PCR of primer progress of step preparation primary PCR amplifies exon 4 and 5 site KIR (killer cell immunoglobulin-like receptors) gene; Step preparation KIR-specific sequences as primer secondary PCR and carry out secondary PCR; With to completely cut off KIR genotype science of heredity;The 2012 of copyright KIPO submissions