Detection of cyprinid herpesvirus type 3 in goldfish cohabiting with CyHV-3-infected koi carp (Cyprinus carpio koi).

摘要

The aim of this study was to detect Cyprinid herpesvirus type 3 (CyHV-3) in goldfish that were cohabiting with CyHV-3-infected koi carp. Three different commercial populations of goldfish which cohabited with koi carp infected with CyHV-3 were included; the first had 38 koi and 80 goldfish, the second 200 small koi and ~50 goldfish, and the third 500 fish of each species. After 4 weeks of cohabitation, all the koi carp died and were found positive for CyHV-3 by PCR. Two weeks later, 15 goldfish (5 from each population) were sampled. LAMP assay indicated that 6 of the 15 samples were positive for CyHV-3. PCR amplified the predicted 409-bp fragment of the TK gene of CyHV-3 in the 6 samples positive by LAMP assay.