Effects of Prostaglandin F_(2α) and Nitric Oxide on the Secretory Function of Bovine Luteal Cells

摘要

The objective of the present study was to investigate the influence of prostaglandin F_(2α) (PGFaa) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle witha nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (N~G-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGF_(2α) (10~(-7)-10~(-5) M), production of progesterone (P4) increased significantly at all doses used (P<0.05). Moreover, PGF2a stimulated PGF2a production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E_2 (PGE_2). Spermine NONOate decreased P_4 production to 66%, 47% and34% of the control concentration after treatment with 10~(-5) M, 10~(-4) M and 10~(-3) M, respectively, but did not affect T production, and increased PGF_(2α) synthesis (P<0.05) and PGE_2 (P<0.01) at all doses used. L-NAME increased production of P4 (P<0.01) but did not affect (P>0.05) secretion of T, PGF2a and PGE2. Estradiol-17β (E_2) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF_(2α) and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.