Tissue accumulation and urinary excretion of chromium in rats fed diets containing graded levels of chromium chlorideor chromium picolinate

摘要

To attempt a risk assessment of the excess intake of trivalent chromium (Cr), tissue Cr accumulation and urinary Cr excretion were examined in weanling rats fed experimental diets containing graded levels of Cr chloride (CrCl_3) or Cr picolinate (CrPic). Thirty-six male weanling 4-weeks-old Wis-tar rats were divided into six groups and fed a casein-based semi-purified diet (Cr content: < 0.02 mug/g) supplemented with 1, 10, or 100 mug Cr/g as CrCl_3 or CrPic for 28 days. Among the experimental groups, no significant difference was observed in body weight; however, supplementation of 100 mug Cr/g to the diets caused a significant low liver weight irrespective of the chemical species of Cr. Activities of serum aspartate aminotransferase and alanine aminotransferase were significantly elevated in rats given CrPic at 100 mug Cr/g. In the liver, kidney and femur, Cr accumulation increased with elevation of the dietary Cr level. No influence of the difference in the chemical species of supplemented Cr was observed in the liver and kidney, but CrCl_3 caused significantly higher Cr accumulation than CrPic in the femur of rats given 100 mug Cr/g. Daily urinary Cr excretion elevated with the increase of the dietary Cr level. Rats given CrPic showed significantly higher daily urinary Cr excretion than those given CrCl_3, particularly at a dietary Cr level of 100 mug/g. The rate of urinary Cr excretion in rats given CrPic was constant, irrespective of the dietary Cr level, but that of rats given CrCl_3 fell with the increase of the dietary Cr level. These results indicate that the lowest adverse effect level of dietary Cr is less than 100 mug/g, irrespective of the chemical species of Cr.