Expression of Fanconi Anemia D2 Gene and its Function in Hela and MCF10A Cells

摘要

Fanconi Anemia (FA) is a disease with autosomal recessive inheritance and characterized by developmental abnormalities, progressive bone marrow failure and cancer predisposition. Especially, the phenotypes of FA cells show the extreme sensitivity towards oxygen and DNA crosslinking agents such as diepoxybutane and mitomycin C (MMC). In the current study, I made the retroviruses which expressed FANCD2 gene for making the stable cell lines, Hela (cervical carcinoma) and MCF10A (breast). I could detectthe expression of FANCD2 protein in the Hela and MCF10A stable cells after the puromycin selection. When I incubated the cells lines with crosslinking agents, MMC, the MCF10A cells which expressed the exogenous FANCD2 was resistant to the MMC, compared with the MCF10 parent cells. But the Hela cells which expressed the FANCD2 did not show the resistance to the MMC. Together, I conclude that FANCD2 protein is an important factor for resistance to the crosslinking agent, MMC in the MCF10A breast cell, butnot the Hela cervical carcinoma cells.