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首页> 外文期刊>Chemistry of Natural Compounds >EFFECT OF DEGLYCOSYLATED EXTENSIN-LIKE PROTEIN ON CELL CULTURE
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EFFECT OF DEGLYCOSYLATED EXTENSIN-LIKE PROTEIN ON CELL CULTURE

机译:葡糖基化的extexin-like蛋白对细胞培养的影响

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Extensin-like proteins(ELP)participate in the construction of plant cell walls,are implicated in interactions between cells connected with molecular recognition,and exhibit protective functions similar to lectins [1,2].Therefore,it seemed interesting to study the role of ELP in cell culture with respect to structure-function relationships.ELP were isolated from 2-day cotton sprouts by extraction with CaCl_2(0.2 M).The resulting proteins were characterized by electrophoresis.The quantitative content of total sugars was determined by spectrophotometry using the anthrone-H_2SO_4 method [3].The carbohydrate content in the isolated samples was 25%.
机译:延伸蛋白样蛋白(ELP)参与植物细胞壁的构建,参与与分子识别相关的细胞之间的相互作用,并表现出与凝集素相似的保护功能[1,2]。因此,研究植物生长素的作用似乎很有趣。细胞培养中ELP的结构-功能关系。通过用CaCl_2(0.2 M)萃取从2天的棉芽中分离出ELP,通过电泳对所得蛋白质进行表征。使用分光光度法测定总糖的定量含量Anthrone-H_2SO_4方法[3]。分离样品中的碳水化合物含量为25%。

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