methods for producing a recombinant protein in a host cell, for releasing recombinant protein expressed by a host cell culture, for reducing the viscosity of a cell culture expressing a recombinant protein, for increasing the extracellular concentration of a recombinant protein expressed by cell culture and for reducing the time period for culturing a cell culture expressing a recombinant protein, pharmaceutical composition, and pei use.

摘要

The present invention relates to a method of producing a recombinant protein in a host cell comprising adding polyethyleneimine (pII) during cell culture. The addition of pei to cell culture as a fermentation enhancer may result in reduced cell culture viscosity and / or increase extracellular concentration of recombinant protein and / or reduce cell culture duration to culture point or protein recovery. .