Separation of Phenolic Acids in Soil and Plant Tissue Extracts by Co-Electroosmotic Capillary Electrophoresis with Direct UV Detection

摘要

A capillary zone electrophoretic method lor the analysis of phenolic adds in soil and plant ex" tractswosdeveloped with direct I.Ndetectiorlusing a ph6sphateelectrolyte sol.vtion. The above thelipKa In order to achieveJheirmlgration towar the anode.Electroosmctic.flow (EOF) was reversed in direction by adding telradecyjtrimethylammonium brom1de ,nAB). Foctorsaffect!ngthe se:.. paratlon selectivity. including the buffer pH andEOF rriodiliers.wereinvestigated systemati- calf~Eightphenolic acids were separotedanddetected in:1Ominusingan electroyte con- tainfng25mMphosphate.0.5mM.TTAB and)5% dcetcnitrite(vlV)qtpHcf Z20.lineQrplots for the test phenolic ocidswere obtained in a concent(al1onrbngeof o.br- hrriM withdetec- lion limits in the rongeof 1.0-7.0 #mu#M. The recover!es ranged!rom,92.810102.3% In soli and pl(JfJf tissues samples spiked of)OO /IMand thf:felotive standard deviationbosed onthe peak area were ranged 2.0 to 4.5%.The proposed method was used for the determination of phenolIc adds in plonHissueand solr.extroctswith direct injection.