Phacoemunification probe as a source of postoperative endophthalmitis following phacoemul-sification cataract extraction (PKE) surgery-DNA sequencing-based study

摘要

A patient who had undergone phacoemulsification cataract surgery presented with redness, pain, diminished vision and hypopyon on the fourth postoperative day. A clinical diagnosis of acute postoperative endophthalmitis was made. As part of its management, an anterior chamber (AC) tap was collected for microbiological investigation. The cytospun smear of this specimen showed Gram-negative bacilli and pus cells. Polymerase chain reaction (PCR) tests for the detection of eubacter-ial, panfungal and Propionibacterium acnes genomes were performed with DNA extracted from the sample (Bio Gene Reagents Inc., CA, USA) using the protocol described previously.