首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Overexpression of AT14A confers tolerance to drought stress-induced oxidative damage in suspension cultured cells of Arabidopsis thaliana
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Overexpression of AT14A confers tolerance to drought stress-induced oxidative damage in suspension cultured cells of Arabidopsis thaliana

机译:AT14A的过表达赋予拟南芥悬浮培养细胞对干旱胁迫诱导的氧化损伤的耐受性

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摘要

Drought stress can affect interaction between plant cell plasma membrane and cell wall. Arabidopsis AT14A, an integrin-like protein, mediates the cell wall-plasma membrane-cytoskeleton continuum (WMC continuum). To gain further insight into the function of AT14A, the role of AT14A in response to drought stress simulated by polyethylene glycol (PEG-6000) in Arabidopsis suspension cultures was investigated. The expression of this gene was induced by PEG-6000 resulting from reverse transcription-PCR, which was further confirmed by the expression data from publically available microarray datasets. Compared to the wild-type cells, overexpression of AT14A (AT14A-OE) in Arabidopsis cultures exhibited a greater ability to adapt to water deficit, as evidenced by higher biomass accumulation and cell survival rate. Furthermore, AT14A-OE cells showed a higher tolerance to PEG-induced oxidative damage, as reflected by less H2O2 content, lipid peroxidation (malondialdehyde (MDA) content), and ion leakage, which was further verified by maintaining high levels of activities of antioxidant defense enzymes such as ascorbate peroxidase and guaiacol peroxidase and soluble protein. Taken together, our results suggest that overexpression of AT14A improves drought stress tolerance and that AT14A is involved in suppressing oxidative damage under drought stress in part via regulation of antioxidant enzyme activities.
机译:干旱胁迫会影响植物细胞质膜与细胞壁之间的相互作用。拟南芥AT14A,一种整合素样蛋白,介导细胞壁-质膜-细胞骨架连续体(WMC连续体)。为了进一步了解AT14A的功能,研究了AT14A在拟南芥悬浮培养物中模拟由聚乙二醇(PEG-6000)引起的干旱胁迫中的作用。该基因的表达是由逆转录-PCR产生的PEG-6000诱导的,这一点进一步得到了公开可得的微阵列数据集的表达数据的证实。与野生型细胞相比,拟南芥培养物中AT14A(AT14A-OE)的过表达表现出更大的适应水分缺乏的能力,这由更高的生物量积累和细胞存活率所证明。此外,AT14A-OE细胞对PEG诱导的氧化损伤表现出更高的耐受性,这表现为较少的H2O2含量,脂质过氧化(丙二醛(MDA)含量)和离子泄漏,这通过保持高水平的抗氧化剂活性进一步得到证实防御酶,如抗坏血酸过氧化物酶和愈创木酚过氧化物酶和可溶性蛋白。两者合计,我们的结果表明,AT14A的过表达提高了干旱胁迫的耐受性,并且AT14A参与了部分干旱胁迫下抗氧化酶活性的调节,从而抑制了干旱胁迫下的氧化损伤。

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