Over-expression of a NAD-Malic Enzyme Gene from Rice in Arabidopsis thaliana Confers Tolerances to Several Abiotic Stresses

摘要

In order to investigate the role of a rice NAD-malic enzyme gene (OsNAD-ME1) under different abiotic stresses,OsNAD-ME1 was constructed into plant expression vector and transformed into Agrobacterium for infecting Arabidopsis thaliana.There were higher transcriptional levels of OsNAD-ME1 in homozygous transgenic lines compared with WT plants as well as higher NAD-ME activity.WT and transgenic Arabidopsis were treated with NaCl,NaHCO3,mannitol and H2O2.And then their root lengths,crown widths and fresh weights were measured and compared.The results showed that over-expression of OsNAD-ME1 in transgenic Arabidopsis increased the resistance to abiotic stresses,which indicated that OsNAD-ME1 was related to stress tolerance.