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Outer membrane protein profiles of Indian isolates of Pasteurella multocida serotype B:2

机译:印度多杀性巴氏杆菌血清型B:2的印度分离株的外膜蛋白谱

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摘要

Outer membrane proteins (OMPs) of 36 isolates of Pasteurella multocida serotype B:22 belonging to buffalo, cattle, pig. sheep and goat including a vaccine strain (P52) were isolated by the sarcosyl extraction method. The size of OMP of P multocida serotype 13:2 isolates and the vaccine strain ranged between 18 and 93 kDa in SDS-PAGE. Some variations were observed in few isolates with respect to OmpA. OmpH and other minor proteins. Out of 36 isolates of P. multocida, 99.44% of isolates belonged to OMP type 1 (OmpA similar to 37 kDa, OmpH similar to 32 kDa) while, 5.56% of isolates represented the OMP type 2 (OmpA similar to 37 kDa, OmpH similar to 36.5 kDa.). Comparison of P. multocida serotype B:2 OMP profiles from different species showed little diversity.
机译:属于水牛,牛,猪的B型多杀巴斯德氏菌血清型B:22的36种分离物的外膜蛋白(OMP)。通过肌糖蛋白提取法分离出包括疫苗株(P52)的绵羊和山羊。在SDS-PAGE中,多杀性疟原虫血清型13:2分离物和疫苗株的OMP大小在18至93kDa之间。关于OmpA,在少数分离物中观察到一些变异。 OmpH和其他次要蛋白质。在多杀性疟原虫的36个分离株中,有99.44%的分离株属于OMP 1型(OmpA类似于37 kDa,OmpH类似于32 kDa),而5.56%的分离株代表OMP 2型(OmpA类似于37 kDa,OmpH类似于36.5 kDa。)。比较来自不同物种的多杀性巴氏杆菌血清型B:2 OMP图谱显示出很少的多样性。

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