QUANTAL RESPONSES TO INOSITOL 1,4,5-TRISPHOSPHATE ARE NOT A CONSEQUENCE OF CA2+ REGULATION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS

摘要

Submaximal concentrations of inositol 1,4,5-trisphosphate (InsP(3)) rapidly release only a fraction of the InsP(3)-sensitive intracellular Ca2+ stores, despite the ability of further increases in InsP(3) concentration to evoke further Ca2+ release. The mechanisms underlying such quantal Ca2+ mobilization are not understood, but have been proposed to involve regulatory effects of cytosolic Ca2+ on InsP(3) receptors. By examining complete concentration-effect relationships for InsP(3)-stimulated Ca-45(2+) efflux from the intracellular stores of permeabilized hepatocytes, we demonstrate that, at 37 degrees C, responses to InsP(3) are quantal in Ca2+-free media heavily buffered with either EGTA or BAPTA [1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid]. Lower concentrations of InsP(3) were used to examine the kinetics of Ca2+ mobilization at 2 degrees C, because at the lower temperature the stores were more sensitive to InsP(3): the concentration of InsP(3) causing half-maximal Ca2+ release (EC(50)) after a 30 s incubation decreased from 281 +/- 37 nM at 37 degrees C to 68 +/- 3 nM at 2 degrees C. At 2 degrees C, the EC(50) for InsP(3)-stimulated Ca2+ mobilization decreased as the duration of exposure to InsP, was increased: the EC(50) was 68 +/- 3 nM after 30 s, and 29 +/- 2 nM after 420 s. InsP(3)-stimulated Ca2+ mobilization is therefore non-quantal at 2 degrees C: InsP(3) concentration determines the rate, but not the extent, of Ca2+ release. By initiating quantal responses to InsP(3) at 37 degrees C and then simultaneously diluting and chilling cells to 2 degrees C, we demonstrated that the changes that underlie quantal responses do not rapidly reverse at 2 degrees C. At both 37 degrees C and 2 degrees C, modest increases in cytosolic Ca2+ increased the sensitivity of the stores to InsP(3), whereas further increases were inhibitory; both Ca-2+ effects persisted after prior removal of ATP. We conclude that the effects of Ca2+ on InsP(3) receptors are unlikely either to be enzyme-mediated or to underlie the quantal pattern of Ca2+ release evoked by InsP(3).