替米沙坦对糖尿病肾病大鼠肾组织Ⅰ型1,4,5-三磷酸肌醇受体表达的影响

摘要

目的:探讨替米沙坦对糖尿病肾病(DN)大鼠肾组织I型1,4,5-三磷酸肌醇受体(IP3R)蛋白及mRNA表达的影响.方法:50只SD大鼠分为5组,即正常对照组(NC组)、DN 6周组(D6组)、DNl2周组(D12组)、替米沙坦治疗6周组(T6组)和替米沙坦治疗12周组(T12组),各组10只.D6、T6、D12和T12组大鼠制作DN动物模型,造模成功后T6和T12组分别给予替米沙坦治疗6和12周.采用免疫组织化学SP法检测各组大鼠肾组织I型I P3R蛋白的表达,RT-PCR测定其mRNA的表达.结果:5组大鼠.肾组织I型IP3R蛋白和mRNA表达的比较.差异有统计学意义(F=42.321和59.482,P均<0.001);T6、D6、T12和D12组较NC升高,T6组较D6组下降,T12组较D12组下降(P均<0.05).结论:替米沙坦可能通过降低I型IP3R的表达治疗DN.%Aim: To detect the expression of type I inositol 1 ,4,5-triphosphate receptors(IP3R) protein and mRNA in kidney tissues of rats with diabetic rephropathy. Methods; A total of 50 SD rats were divided into 5 groups; normal control (NC group) , DN without treatment for 6 weeks( D6 group) ,DN for 12 weeks( D12 group) ,and telmisartan treatment for 6 weeks(T6 group) ,and telmisartan treatment for 12 weeks(T12 group). Immunohistochemistry and reverse transcription PCR(RT-PCR) were used to detect the protein and mRNA expression of type I IP3R in the kidney tissues. Results: The protein and mRNA expression of IP3R type among 5 groups had significant differences ( F =42. 321 and 59. 482, P < 0.001). The expression of type I 1P3R were higher in groups T6,D6,T12 and D12 than that in group NC;compared with that in group D6,it decreased in group T6;comopared with that in group D12,it decreased in group T12(P <0.05). Conclusion : Telmisartan could treat DN through decreasing the expression of type I IP3R in the kidney.