Detection of hydrogen peroxide in rainwater based on Mg-Al-carbonate layered double hydroxides-catalyzed luminol chemiluminescence

摘要

Using a green catalyst of luminol chemiluminescence (CL), Mg-Al-carbonate layered double hydroxides (denoted as Mg-Al-CO_3 LDHs), a novel, sensitive and rapid CL method was developed for the determination of hydrogen peroxide (H_2O_2). The corresponding linear regression equation was established in the range of 0.05-10 μM for H_2O _2. The detection limit (S/N = 3) is 0.02 μM and the relative standard deviation (RSD) for nine repeated measurements of 1.0 μM H _2O_2 was 2.9%. This proposed method has been successfully applied to detect H_2O_2 in rainwater samples with good accuracy and precision. The novel methodology is expected to provide a general protocol for the determination of H_2O_2 as well as for numerous other oxidase-based reactions giving H_2O_2 as a product (e.g., glucose).