Short report: Dengue virus serotype 1 non-structural protein NS5 expression interferes with HIV replication in a CD4+ T-cell line

摘要

The NS5 protein expression of certain flaviviruses, such as Dengue virus type 2, West Nile virus, Hepatitis C virus, and GB virus type C, is associated with a reduced in vitro human immunodeficiency virus (HIV) viral load. To evaluate the suppression effect of the dengue virus serotype 1 (DENV1) NS5 protein on HIV replication, a CD4+ T-cell line was transfected with a plasmid that contained the coding sequence of the DENV1 NS5 gene, and later, it was infected with HIV. DENV1 NS5 protein expression suppressed HIV replication by 28.88% (P < 0.005) on the fifth day compared with the control cells. These results confirm the suppression effect on HIV replication when the DENV1 NS5 protein is expressed in CD4+ T cells. Additional studies are needed to understand exactly how the DENV1 NS5 protein participates in the inhibition of HIV in vitro.