Expression of the Dengue Virus Type 2 NS5 Protein in a CD4+ T Cell Line Inhibits HIV Replication

摘要

Human immunodeficiency virus (HIV) load is suppressed during dengue virus infection. The NS5A phosphoprotein of GB virus C (a related flavivirus) inhibits HIV replication in vitro. To determine whether the dengue virus NS5 protein inhibits HIV replication, CD4+ T cell lines expressing this protein were generated. HIV replication in dengue virus NS5–expressing cells decreased by >90% compared with control cells ( ), and this was mediated in part by decreased HIV coreceptor (CXCR4) expression and increased production of SDF‐1. Thus, the dengue virus NS5 protein inhibits HIV replication in vitro, potentially explaining the reduction in HIV load observed during acute dengue virus infection.