Purification of prostate-specific membrane antigen using conformational epitope-specific antibody-affinity chromatography

摘要

Prostate-specific membrane antigen (PSMA) is a type 11 membrane protein that has attracted significant attention as a target for immunioscintigraphic and radioimmunotherapeutic applications for prostate cancer. However, definitive studies on its substrate and inhibitor specificity as well as protein-protein interactions have been somewhat limited by difficulties in the purification of native PSMA. In this study, we optimized the purification of native PSMA from LNCaP cells using conformational epitope-specific antibody-affinity chromatography. Western blot analysis and an HPLC-based enzymatic activity assay were used to compare the yield and activity of PSMA purified by different methods. The ratio of purified PSMA in a native and active conformation was determined by quantifying the amount of non-native PSMA not retained in a second antibody-affinity isolation. The addition of both a neutralization step and the inclusion of Zn2+ to the equilibration buffer in desalting step provides considerable enhancement in the yield of active PSMA from LNCaP cells. (c) 2006 Elsevier Inc. All rights reserved.